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. 2014 Oct 11;42(19):12331. doi: 10.1093/nar/gku904

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© The Author(s) 2014. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — Bar graphs showing the number of high quality mass spectra for each amplicon length (two panels on the left). The panels on the right side show the corresponding standard deviations of the quantitative measurements. The bar graphs show results for different bisulfite incubation protocols. The results from 16 h incubation at constant temperature are shown in the upper two panels and results from a cycled incubation protocol are shown in the lower two panels. A total of 18 reactions were performed for each amplicon. Cycled incubation and lower incubation temperatures result in higher amplification success for longer amplicons and lower standard deviations on the determination of methylation ratios.