Table 1. PCR primer pair sequences for selected genes.
| Gene | Forward primer | Reverse primer | RT-PCR | qRT-PCR | ChIP |
|---|---|---|---|---|---|
| C8orf4 | CTATGTTTCTACAGGATTTGTAC | CCATATTCATTTCCTAATGCTTAC | x | ||
| c-fos | TTCCTGGCAATAGCGTGTTC | TTCAGACCACCTCGACAATG | x | x | |
| c-jun | TGTGCCCCAAGAACGTGAC | CCGGGTTGAAGTTGCTGAG | x | x | |
| Dusp2 | TGTGGAAATCTTGCCCTACCT | CCCACTATTCTTCACCGAGTCTA | x | ||
| Egr1 | AGCGAACAACCCTATGAGCAC | TCGTTTGGCTGGGATAACTCG | x | x | |
| Egr1 (uncleaved) | GTACTTGTGTTTGCTTAAACAAAGTAAC | ACCACACATGGGTAAGGACTCA | x | ||
| Errfi1 | GCTGCTCAGGATATTCGAGTC | CCAACAGTTGTTAGGTGCTCC | x | ||
| Gapdh | AGGTCGGTGTGAACGGATTTG | TGTAGACCATGTAGTTGAGGTCA | x | ||
| Gapdh (uncleaved) | AGCATCTCCCTCACAATTTCCATC | GTTGATTGAGCCTGCTTCACCTC | x | x | |
| Hes1 | CCAGCCAGTGTCAACACGA | AATGCCGGGAGCTATCTTTCT | x | ||
| Id1 | CTGAACTCGGAGTCTGAAGT | ACTTTTTTCCTCTTGCCTCCT | x | x | |
| Id1 (uncleaved) | GGTCACATTTCGTGCTTCTCG | AAACACTCATTCAGGTCGGTAAG | x | ||
| Id3 | CGCATCTCCCGATCCAGACA | CTGGGTTAAGATCGAAGCTCATCC | x | x | |
| Id3 (uncleaved) | CAGGAAGGTGACTTTCTGTAATC | CAGCCCTTCCTACTAACCAAG | x | x | |
| Id3 3‘UTR | CTGATTATGAACTCTATAATAG | CAAAGTGTTCAAAAATGGTTTATT | x | x | |
| Id3 Promoter | CCTCCAGAAAAGGCATATTC | GAATGAGGAAGCGCTGATAC | x | x | |
| Ier2 | TTTGAGCGACGGTAGTGATGC | GAGACTGGAGAAGCGCCTTTG | x | x | |
| Ier2 (3′UTR) | CTGGTCGTAGTTGCTGCCGTAG | GCGTTTTACACCGATGGTCTTTATTTTCC | x | x | |
| Ier2 (Promoter) | TGCGGAAAACTGGGAGATCTTTAAC | GATTATACTATGGCGAAAGGATCAAC | x | x | |
| Ier2 (uncleaved) | CTGGTCGTAGTTGCTGCCGTAG | CAGTCAAACCAGTTCCGGAG | x | ||
| Lif | GCTGTATCGGATGGTCGCATA | CACAGACGGCAAAGCACATT | x | ||
| Myc | GCTGGATTTCCTTTGGGCGT | CGCAACATAGGATGGAGAGCA | x | ||
| Myc (2071–2245) | GGCTTTGGGACTGTAAGCTTCAGC | GGCCCTATTTACATGGGAAAATTGGATAG | x | ||
| Myc (2071–2399) | GGCTTTGGGACTGTAAGCTTCAGC | GTATTTTTTCCAATTATTTTATTTTTTTCTAAAAAC | x | ||
| Smad7 | GCATTCCTCGGAAGTCAAGAG | CCAGGGGCCAGATAATTCGT | x | ||
| Smad7 (3977–4128) | GCTCGCTCGTATGATACTTTGAC | CCTTTCCTCTCTCAAAGCACTAC | x | ||
| Smad7 (3977–4389) | GCTCGCTCGTATGATACTTTGAC | CATTCAGCTAGGTGATAACACCCA | x | ||
| Snai1 | CAAGGAGTACCTCAGCCTGG | GGTCAGCAAAAGCACGGTT | x | ||
| Wnt11 | GGTGGTACACCGGCCTATG | TCACTGCCGTTGGAAGTCTTG | x | x | |
| Wnt11 (unlceaved) | CTGTTTGTGATGTCTGCCAATAG | GAAGATCTGCCTAAGACACGAAAG | x | ||
| Zfp36 | CATGGATCTCTCTGCCATCTAC | GAGCCAAAGGTGCAAAACCA | x |
C8orf4 (NM_026931.2): RIKEN cDNA 1810011O10 gene (191nt); c-fos (NM_010234.2): FBJ osteosarcoma oncogene (169 nt); c-jun (NM_010591.2): jun proto-oncogene (243 nt); Dusp2 (NM_010090.2): dual specificity phosphatase 2 (232 nt); Dusp2 (NM_010090.2): dual specificity phosphatase 2 (232 nt); Egr1 (NM_007913.5): early growth response 1 (100 nt); Egr1 (uncleaved) (229 nt); Errfi1 (NM_133753.1): ERBB receptor feedback inhibitor 1 (182 nt); Gapdh (NM_008084.2): glyceraldehyde-3 phosphate dehydrogenase (123 nt); Gapdh (uncleaved) (176 nt); Hes1 (NM_008235.2): hairy and enhancer of split 1 (166 nt); Id1 (NM_010495.3): Inhibitor of DNA binding 1 (203 nt); Id1 (uncleaved) (285 nt); Id3 (NM_008321.2): Inhibitor of DNA binding 3 (202 nt); Id3 (uncleaved) (214 nt); Id3 (3′UTR) (139 nt); Id3 (Promoter) (116 nt); Ier2 (NM_010499.4): immediate early response 2 (173 nt); Ier2 (3′UTR) (174 nt); Ier2 (Promoter) (167 nt); Ier2 (uncleaved) (261 nt); Lif (NM_008501.2): leukemia inhibitory factor (156 nt); Myc (NM_001177352.1): myelocytomatosis oncogene (274 nt); Myc (2071–2245) (175 nt); Myc (2071–2399) (329 nt); Smad7 (NM_001042660.1): SMAD family member 7 (225 nt); Smad7 (3977–4128) (152 nt); Smad7 (3977–4389) (413 nt); Snai1 (NM_011427.2): snail family zinc finger 1 (179 nt); Wnt11 (NM_009519.2): wingless-related MMTV integration site 11 (183 nt); Wnt11 (uncleaved) (215 nt); Zfp36 (NM_011756.4): zinc finger protein 36 (218 nt).