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. 2014 Oct 7;42(19):12027–12040. doi: 10.1093/nar/gku913

Figure 3.

Figure 3.

WRN stimulates the pre-steady-state rate constant kpol for dCMP insertion opposite 8-oxo-dG by hpol κ. (A) hPol κ-catalyzed (25 nM) incorporation of dCTP opposite 8-oxo-dG-containing p/t-DNA (50 nM) was performed in the presence of WRN1-1432 (250 nM) and varying concentrations of dCTP (0.5-1000 μM). The product curves were fit to Equation (2) except for the experiment with 0.5 μM, which was fit to Equation (1). The observed rate of product formation (kobs) was plotted as a function of [dCTP] for experiments with hpol κ alone (•) and for experiments with hpol κ performed in the presence of WRN1-1432. The resulting kinetic parameters are listed in Table 4. (B) hPol κ-catalyzed (25 nM) incorporation of dATP opposite 8-oxo-dG-containing p/t-DNA (50 nM) was performed in the presence of WRN1-1432 (250 nM) and varying concentrations of dATP (0.5-500 μM). The product curves were fit to Equation (2). The observed rate of product formation (kobs) was plotted as a function of [dATP] for experiments with hpol κ alone (•) and for experiments with hpol κ performed in the presence of WRN1-1432. The resulting kinetic parameters are listed in Table 4.