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. 2014 Oct 7;42(19):12027–12040. doi: 10.1093/nar/gku913

Figure 5.

Figure 5.

Model for WRN-dependent changes in hpol κ activity on 8-oxo-dG-modified template DNA. (A) The kinetic proficiencies of nucleotide selection by hpol κ at the insertion and extension steps are summarized [the position of 8-oxo-dG in the template is denoted with G in both panels (A) and (B)]. In isolation, hpol κ is ∼70-fold more efficient at error-prone insertion of dAMP opposite 8-oxo-dG and extension from dA:8-oxo-dG mis-pairs than it is error-free bypass of the lesion. (B) The kinetic proficiencies of nucleotide selection by hpol κ at the insertion and extension steps in the presence of WRN are summarized. The combined stimulation of accurate bypass by the WRN RQC domain and preferential degradation of dA:8-oxo-dG mis-pairs by WRN exo result in a slight (1.3-fold) preference for error-free bypass of 8-oxo-dG.