Table 2. Steady-state kinetic parameters of accurate base insertion by hpol κ opposite 8-oxo-dG in the presence of WRN.
dCTP opposite 8-oxo-dG | kcat (min−1) | KM,dNTP (μM) | kcat/KM,dNTP (min−1 μM−1) |
---|---|---|---|
No WRN | 12.4 ± 0.7 | 23.4 ± 3.1 | 0.5 |
WRN1-1432 | 38.5 ± 2.2 | 7.4 ± 1.7 | 5.2 |
WRN1-1092/E84A | 36.3 ± 0.8 | 6.3 ± 0.6 | 5.8 |
WRN1-949/E84A | 12.4 ± 1.6 | 42.9 ± 13.0 | 0.3 |
WRN500-1150 | 35.3 ± 3.5 | 16.2 ± 5.4 | 2.2 |
WRN949-1092 | 34.5 ± 4.2 | 12.7 ± 5.6 | 2.7 |
WRN1-333 | 10.8 ± 0.2 | 21.5 ± 1.6 | 0.5 |
Kinetic parameters were obtained as indicated in Table 1.