Table 4. Pre-steady-state kinetic parameters for nucleotide insertion opposite 8-oxo-dG by hpol κ in the presence and absence of WRN.
| DNA substrate | kpol (s−1) | Kd,dNTP (μM) | kpol/Kd,dNTP (s−1 μM−1) | |
|---|---|---|---|---|
| No WRN | dCTP:8-oxo-dG | 1.5 ± 0.1 | 100 ± 30 | 0.015 |
| WRN1-1432 | dCTP:8-oxo-dG | 9.1 ± 1.1 | 149 ± 61 | 0.061 |
| No WRN | dATP:8-oxo-dG | 4.4 ± 0.3 | 35 ± 8 | 0.12 |
| WRN1-1432 | dATP:8-oxo-dG | 5.4 ± 0.4 | 42 ± 10 | 0.13 |
The observed rate constant for dNTP insertion (kobs) was plotted as a function of dNTP concentration and fit to a quadratic equation. The standard error of the fit is reported for each value.