Table 5. Steady-state kinetic parameters for next-base extension by hpol κ in the presence and absence of WRN for templates containing either dG or 8-oxo-dG.
| DNA substratea | kcat (min−1) | KM,dNTP (μM) | kcat/KM,dNTP (min−1 μM−1) | |
|---|---|---|---|---|
| No WRN | dC:dG | 13.7 ± 0.4 | 7.0 ± 0.7 | 1.9 |
| WRN1-1432 | dC:dG | 26.6 ± 0.5 | 8.0 ± 0.6 | 3.3 |
| No WRN | dA:dG | 0.10 ± 0.01 | 3.8 ± 1.0 | 0.026 |
| WRN1-1432 | dA:dG | 0.11 ± 0.01 | 4.6 ± 1.5 | 0.024 |
| No WRN | dC:8-oxo-dG | 8.0 ± 2.0 | 140 ± 60 | 0.057 |
| WRN1-1432 | dC:8-oxo-dG | 18.0 ± 1.0 | 130 ± 10 | 0.14 |
| No WRN | dA:8-oxo-dG | 16.5 ± 1.0 | 56 ± 8 | 0.29 |
| WRN1-1432 | dA:8-oxo-dG | 14.0 ± 0.7 | 105 ± 18 | 0.13 |
aAll next-base extension experiments measured dGTP insertion since the next template base was dC. The standard error of the fit is reported for each value.