Skip to main content
. Author manuscript; available in PMC: 2014 Nov 14.
Published in final edited form as: Gastroenterology. 2012 Oct 3;144(1):134–144.e6. doi: 10.1053/j.gastro.2012.09.061

Figure 2. Activation of Hh signaling in tumors of Ptch mutant mice.

Figure 2

(A) In situ hybridization of the Hh target genes Gli1 and (B) Ptch in tumors of Ptchflox/floxLysMcre+/− mice. The 477 bp Ptch probe identifies both wild type and mutant Ptch transcripts, in which exon 7 is spliced to exon 10 due to Cre-mediated deletion of exon 8 and 9. The 250 bp Ptch probe exclusively binds to wild type Ptch transcripts. The Figure demonstrates that the tumors overexpress exclusively mutant Ptch transcripts, since wild type Ptch transcripts were not detected. Scale bar in μm: 200.