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. Author manuscript; available in PMC: 2014 Nov 14.
Published in final edited form as: Gastroenterology. 2012 Oct 3;144(1):134–144.e6. doi: 10.1053/j.gastro.2012.09.061

Figure 4. Tumors of Ptch mutant mice express Pdgfrα but not Kit.

Figure 4

(A) Immunofluorescence analysis of tumors of Ptchflox/floxLysMcre+/− mice using an anti-Pdgfrα (green) and anti-Kit antibody (red). No Pdgfrα/Kit double-positive cells were detected in the tumors. Scale bar 50 μm. (B) Western Blot analysis of tumors (Tu) and normal small intestine (In) of Ptchflox/floxLysMcre+/− mice using anti-Pdgfrα and anti-pPdgfrα (phosphorylated Pdgfrα) antibodies. GIST-T1 and NIH/3T3 cells were used as control samples. (C) qRT-PCR analysis of Pdgf-a, b and c in normal intestine and in tumors. Expression levels in tumors are shown in relation to normal intestine, which was set=1. (black bars: intestine; grey bars: tumors).