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. Author manuscript; available in PMC: 2014 Nov 14.
Published in final edited form as: Circ Res. 2006 Jan 26;98(4):490–498. doi: 10.1161/01.RES.0000205846.46812.be

Figure 4.

Figure 4

Heterologous EL expression Confers PPARα Activation In Response to HDL. Standard PPARα–LBD-GAL4 assays were performed as before but in NIH cells known to lack endogenous EL expression. A, NIH cells transfected with either EL or the empty vector were exposed to increasing concentrations of HDL before PPARα–LBD activation was determined. HDL activates the PPARα–LBD in a concentration-dependent manner but only in EL transfected cells. B, PPARα–LBD assays were performed in NIH cells exposed to HDL (100 µg/mL) and increasing amounts of either EL protein conditioned medium (prepared and quantified from EL adenovirus-expressing cells as described in Methods) or control-transfected medium (50 µL/well to 500 µL/well of medium, 24-well plates). The extent of PPARα-LBD activation seen was linearly related to the amount of EL-conditioned medium added; control medium had no effect.