Figure 6.
EL/HDL induces mRNA expression of the PPARα target gene acyl CoA oxidase (ACO) but only in the genetic presence of PPARα. ECs from PPARα wild-type (+/+) and deficient (−/−) mice were transfected with EL or a control plasmid and stimulated with either WY14643 (10 µmol/L) or HDL (90 or 200 µg/mL) before performing Northern blot analysis for ACO. Densitometry and quantification analysis as compared with GAPDH expression indicates WY and HDL increased ACO 1.92-fold and 2.47-fold, respectively, in EL-transfected cells.