Figure 7.

EL and LPL are distinct mechanisms for lipolytic PPARα activation. The additional PPARα–LBD activation seen with EL vs LPL in response to VLDL or HDL was compared in BAEC. A, Cells were exposed to increasing concentrations of LPL as shown in the presence of a fixed concentration of VLDL (5 µg/mL). As previously reported,¹⁰ LPL increased PPARα activation in a concentration-dependent manner. The presence or absence of EL-transfection had no impact on the PPAR activation seen by LPL/VLDL. B, Similar experiments as in (A) were repeated with increasing concentrations of LPL but in the presence of a fixed concentration of HDL (90 µg/mL). With HDL as the substrate, the presence of EL overexpression significantly increases the PPARα activation seen at any given LPL concentration (P<0.05 for each LPL concentration).