| Method |
Hanging-drop vapour diffusion |
Hanging-drop vapour diffusion |
| Plate type |
24-well plate |
24-well plate |
| Temperature (K) |
295 |
295 |
| Protein concentration (mgml1) |
18 |
18 |
| Buffer composition of protein solution |
50mM TrisHCl pH 7.5, 50mM NaCl |
50mM TrisHCl pH 7.5, 50mM NaCl |
| Composition of reservoir solution |
1.0M sodium citrate, 0.1M HEPES pH 7.5 |
0.2M sodium acetate pH 7.2, 30% PEG 8K |
| Volume and ratio of drop |
2.5l protein solution and 2.5l reservoir solution |
2.5l protein solution and 2.5l reservoir solution |
| Volume of reservoir (l) |
500 |
500 |
