Table 1. DNA constructs used in this study.
DNA fragment† | Sequence‡ | Co-crystals with LANA(9961149) mutant |
---|---|---|
LBS1(017) | 5-ctcccgcccgggcatggg-3 | No |
3-gagggcgggcccgtaccc-5 | ||
LBS1(017) palindrome ‘A’ | 5-cCcATgcccgggcatggg-3 | Thin plates |
3-gGgTAcgggcccgtaccc-5 | ||
LBS1(017) palindrome ‘B’ | 5-ctcccgcccgggcGGgAg-3 | No |
3-gagggcgggcccgCCcTc-5 | ||
LBS1[(2)22] A:T overhang | 5-Agcctcccgcccgggcatggggccgc-3 | No |
3-cggagggcgggcccgtaccccggcgT-5 | ||
LBS1(018) | 5-ctcccgcccgggcatgggg-3 | No |
3-gagggcgggcccgtacccc-5 | ||
LBS1(320) | 5-ccgcccgggcatggggcc-3 | No |
3-ggcgggcccgtaccccgg-5 | ||
LBS1(221) | 5-cccgcccgggcatggggccg-3 | Cubic crystals |
3-gggcgggcccgtaccccggc-5 |
The numbering of the sequence corresponds to that of Srinivasan et al. (2004 ▶).
The 8bp palindrome is underlined. Bases differing from the original sequence are denoted by capital letters.