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. Author manuscript; available in PMC: 2014 Nov 14.
Published in final edited form as: Crit Care Med. 2014 Jun;42(6):e420–e431. doi: 10.1097/CCM.0000000000000309

Figure 6.

Figure 6

Protease activity is altered in distal, unburned skin after local burn injury. A, Gene expression of kallikrein (KLK) 5 and KLK7 in distal skin at 24 and 96 hr postburn. n = 3–6 samples/group. Data analyzed by unpaired t test (*p < 0.05). B, Distal skin was stained by immunohistofluorescence (IHF) for KLK5 and KLK7 at 24 hr postburn. n = 3–4 samples/group and performed in duplicate. Protease activity at 24 hr (C) and 96 hr (D) postburn using the EnzChek Protease Assay. Data expressed as raw fluorescence observed and normalized to protein concentration. n = 6–7 samples/group. Data analyzed by unpaired t test (*p < 0.05). E, Protease activity in distal skin at 24 and 96 hr postburn by IHF at 20× magnification. n = 3–4 samples/group.