Figure 5.

S-phase-dependent G-tail formation in mre11Δ cells. A cell synchronization experiment using MRE11 and mre11Δ cells (AR120 and MLY40 strains) was performed as described in Supplementary Figure 1. DNA isolated from each cell aliquot was analyzed for telomeric DNA end-structures by nondenaturing in-gel hybridization (Supplementary Fig. 2; data not shown). The relative amounts for telomeric ssDNA on YLpFAT10 (in arbitrary units) were obtained by calculating the ratio of the G-tail signals retrieved on the native gel over the total amount of YLpFAT10 detected.