Table 1.
Affymetrix microarray analysis of brain transcripts in double- and triple-knockout mice
| Name | Product/homology | GenBank | Fold change | N/9 |
|---|---|---|---|---|
| A | ||||
| Tef | Thyrotroph embryonic factor | AI850638 | 7.42 ± 1.39 | 9 |
| Nfil3/E4BP4 | Nuclear factor, interleukin 3, regulated | U83148 | 2.59 ± 0.97 | 8 |
| EST (PDXK) | RIKEN cDNA 2310036D04 gene (similar to Pyridoxal Kinase) | AI841777 | 2.05 ± 0.25 | 9 |
| EST | RIKEN cDNA 0910001L24 gene (putative epoxide hydrolase) | AI836883 | 1.88 ± 0.25 | 9 |
| Per2 | Period homolog 2 | AF036893 | 1.73 ± 0.22 | 6 |
| EST | RIKEN cDNA 1200013P24 gene (GCN5-related N-acetyltransferase) | AI846961 | 1.60 ± 0.08 | 9 |
| Csflr | Colony stimulating factor 1 receptor | X06368 | 1.59 ± 0.18 | 7 |
| Bhlhb2/Dec1 | Basic helix–loop–helix domain containing, class B2 (Dec1) | Y07836 | 1.59 ± 0.15 | 8 |
| Baiap2 | Brain-specific angiogenesis inhibitor 1-associated protein 2 | AI844631 | 1.56 ± 0.15 | 8 |
| Igfbp6 | Insulin-like growth factor binding protein 6 | X81584 | 1.52 ± 0.15 | 7 |
| EST | RIKEN cDNA B930035K21 gene | AI846522 | 1.50 ± 0.15 | 8 |
| Cx3cl1 | Chemokine (C-X3-C motif) ligand 1 | U92565 | 1.50 ± 0.19 | 7 |
| B | ||||
| Anp32a | Acidic (leucine-rich) nuclear phosphoprotein 32 family, member A | U73478 | 6.92 ± 5.19 | 7 |
| EST | Murine (DBA/2) mRNA fragment for gag related peptide | X05546 | 3.34 ± 2.51 | 6 |
| EST | Mus musculus prosaposin gene, promoter and exon 1 | AF037437 | 3.06 ± 2.80 | 6 |
| Kif1a | Kinesin heavy chain member 1A | D29951 | 2.54 ± 1.25 | 6 |
| EST | RIKEN cDNA C330012F17 gene | AA189811 | 2.47 ± 0.44 | 8 |
| Gna12 | Guanine nucleotide binding protein, alpha 12 | M63659 | 2.40 ± 1.70 | 6 |
| Sty1 | Synaptotagmin 1 | D37792 | 2.38 ± 1.06 | 6 |
| Timp2 | Tissue inhibitor of metalloproteinase 2 | X62622 | 2.31 ± 1.45 | 6 |
| Kif5a | Kinesin family member 5A | AF053473 | 2.25 ± 1.37 | 6 |
| Atp6v0a1 | ATPase, H+ transporting, lysosomal V0 subunit a isoform 1 | U13836 | 2.14 ± 1.42 | 6 |
| Ptpns1 | Protein tyrosine phosphatase, nonreceptor type substrate 1 | D85785 | 2.09 ± 1.20 | 6 |
| Zfp179 | Zinc finger protein 179 | AB013097 | 1.99 ± 1.37 | 6 |
| Ptpns1 | Protein tyrosine phosphatase, nonreceptor type substrate 1 | D85785 | 1.84 ± 1.07 | 6 |
| Ptn | Pleiotrophin (heparin binding growth factor 8, neurite growth-promoting factor 1) | D90225 | 1.79 ± 0.52 | 6 |
| Hspc121 | Butyrate-induced transcript 1 (HSP20-like chaperone) | Z97207 | 1.71 ± 0.73 | 6 |
| Usp22 | Ubiquitin specific protease 22 | AW125800 | 1.54 ± 0.42 | 6 |
| Ppp1cb | Protein phosphatase 1, catalytic subunit, beta isoform | M27073 | 1.51 ± 0.24 | 8 |
| Vamp2 | Vesicle-associated membrane protein 2 (synaptobrevin 2) | U60150 | 1.50 ± 1.11 | 6 |
| Gria1 | Glutamate receptor, ionotropic, AMPA1 (α 1) | AW048549 | 1.50 ± 0.55 | 6 |
Transcripts that are less abundant or more abundant in triple knockout mice as compared to double knockout mice are listed in panel A and B, respectively. The “Fold change” is the average value ± S.D. of all nine comparisons (even if the values were not different in all nine comparisons). N/9 indicates the number of comparisons in which the hybridization signals were significantly different. Note that Pdxk mRNA yielded a lower hybridization signal with RNA from triple-knockout mice in nine out of nine comparisons, whereas not a single transcript listed in panel B (higher in triple- than in double-knockout mice) shows a difference in all nine comparisons.