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. 2004 Jun 15;18(12):1439–1451. doi: 10.1101/gad.1184204

Figure 2.

Figure 2.

Overexpression of Mad2p reduces Cdc20p levels in the presence of nocodazole. (A) 8mycCDC20, PGAL-pds1Δdb cells containing PGAL vector alone or PGAL-MAD2 (RHC401 and RHC402) were first arrested at G1 with α-factor, then washed and released into YEPR in the presence or absence of nocodazole at 30°C. When ∼10% of the cells began to bud, 2% galactose was added to the medium. Samples were taken at 30-min intervals after galactose addition for immunoblot analysis. Mad1p blots serve as loading controls. (B) Signals of 8mycCdc20p in A were quantified and plotted against time.