Table 3.

Plasmids used in this study

Plasmid	Features	Source
YCplac111	yeast centromeric plasmid with LEU2 selectable marker	Gietz and Sugino 1988
YCplac111-MS2-GFP	MS2–GFP fusion cDNA cloned into YCplac111	Bertrand et al. 1998
YEplac195	yeast multicopy plasmid with URA3 selectable marker	Gietz and Sugino 1988
YEplac195-Gal-ASH1 (f)	YEplac195 containing 6 MS2 binding sites wild-type ASH1 gene under GAL1 promoter	This work
YEplac195-Gal-LacZ-MS2-El	YEplac195 containing LAcZ reporter and 6 MS2 binding sites fused with ASH1 E1 element under GAL1 promoter	This work
YEplac195-Gal-LacZ-MS2-E2A	YEplac195 containing LacZ reporter and 6 MS2 binding sites fused with ASH1 E2A element under GAL1 promoter	This work
YEplac195-Gal-LacZ-MS2-E2B	YEplac195 containing LAcZ reporter and 6 MS2 binding sites fused with ASH1 E2B element under GAL1 promoter	This work
YEplac195-Gal-LacZ-MS2-E3	YEplac195 containing LAcZ reporter and 6 MS2 binding sites fused with ASH1 E3 element under GAL1 promoter	This work
pFastBacHT	Plasmid for protein expression in insect cells	Life Technology
pPuf6-exp	pFastBacHT expression vector containing entire Puf6p ORF	This work
pCDNA3.1	Vector for in vitro transcription	Invitrogen
pCAT-ASH1-3′W	pCDNA3.1 containing entire CAT ORF fused with ASH1 3′UTR	This work
pCAT-ASH1-3′M	pCDNA3.1 containing entire CAT ORF fused with mutant ASH1 3′UTR	This work
pSP64	Vector for in vitro transcription	Promega
pSP64-ASH1-3′W	pSP64 containing wild type ASH1 3′UTR	This work
pSP64-ASH1-3′M1	pSP64 containing mutant ASH1 3′UTR at UUGU-1	This work
pSP64-ASH1 -3′M	pSP64 containing mutant ASH1 3′UTR at UUGU-1 and UUGU-2	This work
pBS 1479	Plasmid for tap-tagging and gene disruption	Rigaut et al. 1999
pXR193	YIPlac128 containing wild-type ASH1 gene with myc9-tag at the 3′ end	Chartrand et al. 2002
pXR252	pXR193 plasmid with a stem-loop in the 5′UTR	Chartrand et al. 2002
pXR193-mut	pXP193 plasmid with the UUGU mutation at the 3′UTR of ASH1 gene	This work