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. 2014 Mar 21;16(8):1255–1266. doi: 10.1111/cmi.12281

Figure 2.

Figure 2

T84 cell integrity and STEC adherence after 5 h infection. Shown are representative images from two independent experiments performed in duplicate (A–C).

A. Confocal XZ scan of T84 cells infected with EDL933. Cell monolayers were stained for E. coli (red), F-actin (green) and cell nuclei (blue). Bar = 5 μm.

B. Immunofluorescence staining of non-infected (NI) and EDL933-infected T84 cells. Cells were stained for occludin (green) and E. coli (red). Bar = 10 μm.

C. Scanning electron micrographs of non-infected T84 cells (NI) and cells infected with EDL933 or H1121. Bars = 5 μm (NI), 2 μm (H1121, left panel) and 1 μm (EDL933; H1121, right panel).

D. TER of non-infected T84 cells (NI) and cells infected with EDL933, Walla-1 or H1121 under AE (■) or MA (□) conditions. TER after infection is expressed as percentage of TER before infection. Data are shown as means ± SEM from seven independent experiments performed in duplicate.