Figure 3.

Microaerobic conditions reduce STEC EDL933 growth and Stx release into the apical medium. T84 cells were infected for 3 to 5 h and maintained under AE (●) or MA (□) conditions. Bacterial growth was assessed by OD₆₀₀ (A). Stx cytotoxicity in apical supernatants was determined by VCCA (B). Data are shown as means ± SEM from three independent experiments performed in duplicate. *P < 0.05, **P < 0.01.