Skip to main content
. 2014 Mar 21;16(8):1255–1266. doi: 10.1111/cmi.12281

Figure 6.

Figure 6

Microaerobiosis reduces growth, Stx production and release in STEC EDL933, Walla-1 and H1121. Polarized T84 cells were infected for 5 h under AE (■) or MA (□) conditions.

A. Bacterial growth was assessed by OD600. Data are shown as means ± SEM from five independent experiments performed in duplicate. **P < 0.01, ***P < 0.001 versus AE conditions; $$P < 0.01 versus EDL933 AE.

B. Stx2 levels in apical supernatants were quantified by ELISA. Data are shown as means ± SEM from four independent experiments performed in duplicate. *P < 0.05, **P < 0.01, ***P < 0.001 versus AE; $$$P < 0.001 versus EDL933 AE; &P < 0.05, &&&P < 0.001 versus EDL933 MA.

C. Stx1 production in bacterial lysates of EDL933 (E), Walla-1 (W) and H1121(H) under AE (A) and MA (M) conditions was determined by Western blotting. Amounts of loaded bacterial protein were assessed by GroEL expression. The marker lane (M) indicates 58 kDa for GroEL and 30 kDa for Stx1. Shown are representative images from three independent experiments.