Figure 7.

Stx2 translocation across polarized T84 cells is enhanced by STEC infection under MA conditions. T84 cells were apically incubated with EDL933 (A, B), H1121 (A) or purified Stx2 (B) for 5 h under AE (■) or MA (□) conditions. Stx2 concentrations in apical and basal media were determined by VCCA. Translocation rates are expressed as percentage of apical Stx recovered in basal compartments.

A. Data are shown as means ± SEM from seven independent experiments performed in duplicate. ***P < 0.001 EDL933 MA versus AE; ^&&P < 0.01 H1121 MA versus EDL933 MA.

B. Data are shown as means ± SEM from five independent experiments performed in duplicate. **P < 0.01 versus AE; ^&P < 0.05 versus EDL933 MA.