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. 2014 Nov 13;55(11):7227–7240. doi: 10.1167/iovs.14-14594

Table.

Total RNA Was Extracted From Mouse Tissues by TRIzol Plus RNA Purification System (Invitrogen)

1 CryαA: F: 5′-gagattcacggcaaacacaa-3′
R: 5′-aaattcacgggaaatgtagcc-3′
2 CryαB: F: 5′-cctgagccccttctaccttc-3′
R: 5′-tccttctccaaacgcatctc-3′
3 Cryγs: F: 5′-gaataccagcgttggatgg-3′
R: 5′-gaaggacagtcttccgtgg-3′
4 Vim: F: 5′-ccaaccttttcttccctgaa-3′
R: 5′-tgagtgggtgtcaaccagag-3′
5 Fgf7: F: 5′-cagacagcagacacggaac-3′
R: 5′-gcctcctcctatttgtgaac-3′
6 EGFP: F: 5′-cttcttcaagtccgccat-3′
R: 5′-caccttgatgccgttcttct-3′
7 Hsf4: F: 5′-gagccacagtcagcagcac-3′
R: 5′-cgctcccctcatctagca-3′
8 Gapdh: F: 5′-ggtgaaggtcggtgttgaacg-3′
R: 5′-ctcgctcctggaagatggtg-3′
9 Hsp25: F: 5′-ggaagtctgaacagtctgga-3′
R: 5′-gtatcaaaagagcgcacag-3′
10 Hsp70: F: 5′-cagtagcctgggaagacata-3′
R: 5′-tagtacacagtgccaagacg-3′
11 Hsp60: F: 5′-cagagttcctcagaagttgg-3′
R: 5′-catccagtaaggcagttctc-3′
12 Bfsp1: F: 5′-acagacaagaatgggttacg-3′
R: 5′-aggtatgatcacaggacagg-3′
13 Bfsp2: F: 5′-ggctttctgtcaagaagcta-3′
R: 5′-ctcaaggacatcatccagac-3′
14 LP82: F: 5′-acacagaccaggaaagtgag-3′
R: 5′-tgtgttgaggacattcttga-3′
15 Calp2: F: 5′-attctggatgtccttcagtg-3′
R: 5′-ttggtgagtttccacttctt-3′
16 Dlad: F: 5′-gacagcaaagcctctaagaa-3′
R: 5′-gtccacagcttcaccatatt-3′

Approximately 1 μg of total RNA was treated by DNase I (Invitrogen) and reverse transcribed by using Superscript III (Invitrogen) in 20 μl. For RT-qPCR, 1 μL of cDNA was amplified with a SYBR Green Master mix for 45 cycles (Hoffmann-La Roche, Basel, Switzerland) and analyzed as described.37 Primers used for RT-qPCR (Figs. 7B, 7C, 8A, 8B) are shown above. F, forward; R, reverse.