Figure 5.

Supraciliary targeting of injections using a high-viscosity formulation injected using a microneedle. (a) Injection of 10 μL red fluorescing particles (1-μm diameter, 0.5% w/v) into the rabbit eye ex vivo spread only a few millimeters from the site of injection. The whole eye was imaged by digital camera 60 min after injection. (b) Injection of 50 μL red fluorescing particles (1-μm diameter, 0.5% w/v) into the rabbit eye ex vivo was localized to the supraciliary space, directly above and adjacent to the ciliary body. The eye was frozen immediately after injection, prepared as frozen sections, and imaged by brightfield microscopy. (c) Injection of 30 μL red fluorescing microparticles (10-μm diameter, 0.5% w/v) into a human cadaver eye was localized to the supraciliary space. The eye was fixed in formalin immediately after injection, prepared as frozen sections, and a brightfield image was overlaid on a fluorescent image to show ocular anatomy with fluorescence in the same histological section.