Figure 3.
Analysis of the ppcA1 GUS Reporter Gene Constructs ppcA-PRFt-DR(+)Ft and ppcA-PRFt-DR(−)Ft in Transgenic F. bidentis.
(A) Structures of the ppcA1/GUS chimerical genes.
(B) GUS activities in leaves of transgenic F. bidentis plants. The numbers of independent transgenic plants investigated (n) are indicated at the top of each column. Median values are shown. Mu, 4-methylumbelliferone.
(C) and (D) Histochemical localization of GUS activity in leaf sections of transgenic F. bidentis plants transformed with the ppcA-PRFt-DR(+)Ft (C) or the ppcA-PRFt-DR(−)Ft construct (D). Incubation time was 20 min in case of the ppcA-PRFt-DR(+)Ft plant and 10 min in case of the ppcA-PRFt-DR(−)Ft plant.