Figure 2. HapX-deficiency renders A. fumigatus more susceptible to iron toxicity than CccA-deficiency and impairs induction of genes involved in iron-consuming processes.

1. Strains were grown on solid minimal medium with the given iron availability under xylP-driven cccA^OE non-inducible (0% xylose) and inducible (0.5% xylose) conditions for 48 h at 37°C.
2. Northern blot analysis was performed after liquid growth for 24 h at 37°C under iron limitation and a subsequent 1-h shift to iron sufficiency (sFe). rRNA is shown as a control for RNA quantity and quality.

Data information: Strains are derivatives of A. fumigatus ATCC 46645.