Skip to main content
. 2014 Aug 4;33(19):2261–2276. doi: 10.15252/embj.201489468

Figure 5. The HapX C-terminus is crucial for adaption to iron starvation but not iron detoxification.

Figure 5

  1. Strains were grown for 48 h at 37°C on agar plates with the given iron concentration.
  2. Production of biomass during iron starvation (−Fe), iron sufficiency (0.03 mM, +Fe), and iron excess (3 mM, hFe), as well as production of siderophores and PpIX under iron starvation was monitored after liquid growth for 24 h at 37°C. The data represent the mean ± SD of biological triplicates; the values were normalized to the wild-type. Statistically significant differences compared to the wild-type are shown in red (two-tailed, unpaired t-test; P < 0.05). Original data ± SD are found in Supplementary Table S1.
  3. Northern blot analyses were performed after liquid growth for 24 h at 37°C under iron limitation (−Fe) or after a subsequent 1-h shift to iron sufficiency (sFe). rRNA is shown as a control for RNA quantity and quality.
  4. Schematic view of the HapX truncations analyzed.

Data information: Strains are derivatives of A. fumigatus AfS77.