Figure 8.

Differential Responses to Osmotic Strengths between SAPK1 and SAPK2 Are Determined by the C-Terminal Domain.

Protoplasts expressing dHA-His–tagged SAPK1 (A) or SAPK2 (B) or a chimeric kinase consisting of the SAPK1 kinase domain and the SAPK2 C-terminal domain (C) or consisting of the SAPK2 kinase domain and the SAPK1 C-terminal domain (D) were treated with NaCl at the indicated concentration for 30 min, analyzed, and quantified as in Figures 3 and 6B. On the top of each panel, the structure of the wild type or chimeric kinase, including the amino acid residues at the borders of the kinase and the C-terminal domains, is illustrated.