Figure 5.

Functional Analysis of Conserved SPR1 Domains.

(A) Schematic pictures of full-length SPR1 coding sequence (top) and four chimeric DNA constructs. 35S, 35S promoter of Cauliflower mosaic virus; GFP, GFP-coding sequence; N, coding sequence for the 23–amino acid N-terminal region of SPR1; C, coding sequence for the 41–amino acid C-terminal region of SPR1; V, coding sequence for the 55–amino acid internal variable region of SPR1; aa, amino acids. See Figure 2D for exact excision points.

(B) Definition of the root skewing angle (θ) listed in Table 1.

(C) to (F) Independent T1 transgenic plants growing on hard agar plates.

(G) to (J) Confocal images of upper hypocotyl regions of dark-grown seedlings.

(C) and (G) P_35S:NG; (D) and (H) P_35S:GC; (E) and (I) P_35S:NGC; (F) and (J) P_35S:G.

Bars in (C) to (F) = 1 cm; bars in (G) to (J) = 10 μm.