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. 2014 Jul 11;16(12):1618–1629. doi: 10.1093/neuonc/nou132

Fig. 2.

Fig. 2.

EFTUD1 expression pattern in glioma cells and tissue analyzed by immunohistochemistry (A) and immunofluorescence (B). (A) Immunohistochemical analysis of EFTUD1 expression in glioma tissue and normal brain tissue. Representative immunohistochemical sections of 2 GBM patient tissues (Patient 1, magnification 10×; Patient 2, magnification 40×) and normal brain tissue (magnification 10×) using an EFTUD1 antibody are shown. The sections were counterstained with hematoxylin. Scale bar, 50 μm. The boxed area shows higher magnification images. Scale bar in the boxed area, 10 μm. (B) Immunofluorescent cell staining of glioma cell lines (U87MG and U251MG) using an EFTUD1 antibody and a rhodamine-conjugated secondary antibody. Cell nuclei were stained with DAPI. Scale bar, 50 μm.