Figure 2.

Suppression of CHS-RNAi by P1-HcPro, P19, P38, P25, and P15.

(A) One day after light induction, total RNA was extracted from leaves of wild-type plants or of line CHS-RNAi crossed or not with the silencing suppressor–expressing lines. Fifteen micrograms of this RNA was subjected to RNA gel blot analysis using a CHS cDNA probe. Anthocyanins were extracted in parallel and quantified by spectrophotometry.

(B) RNA gel blot analysis of low molecular weight RNA (15 μg) extracted before light induction. The hybridization was with a CHS cDNA probe. nt, nucleotides.

(C) Twenty-five micrograms of the RNA used in (B) was treated with RNase A, deproteinized, heat denatured, and subjected to RNA gel blot analysis using a CHS cDNA probe.