Figure 6.

Effects of the Silencing Suppressors on miRNA-Mediated Cleavage of Target mRNAs.

(A) Two possible outcomes of miRNA-guided cleavage of endogenous transcripts in plants. In the first case (1), both the 5′ and 3′ cleavage fragments are degraded, whereas in the second case (2), the 3′ cleaved fragment remains stable. The miRNA is indicated in red.

(B) and (C) Fifteen micrograms of total RNA extracted from inflorescences of the various suppressor-expressing lines were subjected to RNA gel blot analysis using cDNA probes specific for the CUC1 (B) or the SCL6-IV and ARF10 mRNAs (C). The size of the predicted 3′ cleavage products of SCL6-IV and ARF10 [SCL6-IV(b) and ARF10(b), respectively] is indicated. nt, nucleotides.