Figure 7.

Diversity and Complexity of the Arabidopsis Silencing-Related Small RNAs.

(A) RNA gel blot analysis of RNAs extracted from leaves and stems of the plants that were used to produce the inflorescence data presented in Figure 4. RNA gel blot conditions were the same. See also Supplemental Data 3 online for analysis of additional miRNAs. nt, nucleotides.

(B) Total RNA was extracted from inflorescences of wild-type, P1-HcPro class I, and P1-HcPro class III plants and subjected to RNA gel blot analysis. The probes used were labeled oligonucleotides complementary to miR156, miR160, and miR171, respectively. rRNA, ethidium bromide staining of rRNA.

(C) Accumulation of the 24-nucleotide miR163 in leaves and flowers of the suppressor lines.

(D) and (E) Accumulation of small RNA 96 (D) and of small RNAs of the AtSN1 retroelement (E) in flowers from the various suppressor-expressing lines.

(F) Flower-specific accumulation of the 21-nucleotide small RNA2 in inflorescences of the various suppressor-expressing lines. The probes used in (C) to (F) were labeled oligonucleotides complementary to the corresponding small RNAs.