Figure 5.

p69 Does Not Suppress the IR-RNA–Induced PTGS Targeting of PDS.

(A) to (C) Genetic segregation of the photobleaching RNA interference phenotype of the IRPDS transgene after crossing an IRPDS heterozygote (IRPDS/−, in Col-0 background) with either C-24 or a P69c homozygote (in the C-24 background). A representative plate is shown for each except for P69cxIRPDS, which did not show difference with or without hygromycin selection. P69c plants appear yellow in contrast with the photobleached plants (C).

(D) Detection of P69 mRNA and PDS mRNA/siRNAs. For comparison to the level of PDS mRNA in the C24xCol-0 genetic background with and without P69, RNA samples from the progeny of C24xCol-0 (lane 3) and P69cxCol-0 (lane 1) were included as controls. Equal loading was monitored for mRNA by rRNA staining and for small RNAs by reprobing for 5S rRNA.