Figure 4.

mRNA Localization of Predicted Floral Organ–Specific Transcripts.

(A) and (B) Transverse sections through a stage 11 flower. Expression of At1g61110 (NAC-family transcription factor) was detected inside anthers in the tapetal cell layer.

(C) Longitudinal section through a stage 11 flower. At2g07040 (receptor-like kinase) is expressed in pollen.

(D) and (E) Longitudinal sections through stage 10 floral buds. Expression of At2g17950 (WUS) is found in developing ovules (D) and between the locules of anthers (E).

(F) Transverse section through a stage 10 floral bud. At1g75030 (thaumatin-like protein) is expressed in the tapetum.

(G) and (H) Longitudinal (G) and transverse (H) sections through stage 12 flower buds. Expression of At4g12960 (expressed protein) was detected in ovules.

(I) Longitudinal section through a stage 12 flower. At2g43230 (kinase) is expressed in pollen.

(J) and (K) Transverse section (J) and longitudinal section (K) through stage 12 flowers. Expression of At3g61160 (ASKβ) is found in pollen (J) and inside ovules, probably in egg cells (K).

(L) Transverse section through a stage 9 flower. mRNA of At3g26860 (putative self-incompatibility protein) was detected in tetrads during pollen development (indicated by arrows). For all in situ hybridizations, the use of sense probes did not result in signals above background (data not shown).