Table 1.
hma Mutant Alleles Identified in This Study
| Allelea | Ecotype | Positionb | Gene/T-DNA Boundary Sequence (5′/3′)c |
|---|---|---|---|
| hma2-1 | Ws | Intron 1 (257) | CAAACGTCGTTTTCAG/gatatattca |
| hma2-2 | Ws | Exon 6 (2399) | ACTATCACTAGAGGTGA/tatattcaaa |
| T I T R G | |||
| hma2-3 | Ws | Intron 1 (580) | cgtcaatgtgtt/AGGTGGGTTTTTACG |
| hma2-4 | Col | Intron 1 (329) | TATTGAGTTACATCT/taataacaca |
| hma3-1 | Ws | Exon 1 (139) | AAAGAATTCTCAGTCA/attgtaaatg |
| K E F S V | |||
| hma4-1 | Ws | Exon 1 (146) | GGCGTTAAAGAATATT/ctcaggatat |
| G V K E Y | |||
| hma4-2 | Col | Exon 4 (3439) | tcaatttgtt/AGCTCCACAAAAGGC |
| A P Q K |
a
hma2-4 is the SALK_034393 insertion line; hma4-2 is SALK_050924.
b
Numbers indicate the base pair immediately adjacent to the left border of the T-DNA insertion relative to the A of the predicted initiation codon of the respective gene sequence derived from the Col ecotype.
c
The slash identifies the boundary between gene sequence and insertion sequence. Gene sequences are in upper case, intron sequences are underlined, coding sequences are translated below, and insertion sequences are in lower case.