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. 2014 Nov 14;9(11):e110566. doi: 10.1371/journal.pone.0110566

Table 1. Pathogen performance of the multiplexed prototype assay [including discrepant results resolution in brackets].

Sensitivity Specificity
Cultured organisms: TPa FNb FPc TNd [%] [%] PPVe [%] NPVf [%]
Acinetobacter baumannii 2 0 5 [1] 531 100 99.1 28.6 100
Enterobacter spp. 7 3 [2] 55 [14] 397 70 87.8 11.3 99.3
Escherichia coli 19 8 [5] 12 [7] 509 70.4 97.7 61.3 98.5
Haemophilus influenzae 4 0 43 [33] 473 100 91.7 8.5 100
Klebsiella oxytoca 0 1 [0] 5 [1] 335 0 98.5 0 99.7
Klebsiella pneumoniae 10 8 [7] 0 381 55.6 100 100 97.9
Moraxella catarrhalis 0 1 [0] 15 [12] 498 0 97.1 0 99.8
Morganella morganii 0 1 [0] 6 [1] 514 0 98.8 0 99.8
Proteus spp. 4 4 [3] 7 [0] 465 50.0 98.5 36.4 99.1
Pseudomonas aeruginosa 19 10 [6] 6 [3] 306 65.5 98.1 76 96.8
Serratia marcescens 11 1 [1] 6 [6] 529 91.7 98.9 64.7 99.8
Staphylococcus aureus 22 12 [11] 13 [9] 433 64.7 97.1 62.9 97.3
Stenotrophomonas maltophilia 20 1 [1] 46 [28] 471 95.2 91.1 30.3 99.8
Streptococcus pneumoniae 2 0 98 [69]* 414 100 80.9 2 100
Total 120 50 [36] 317 [184] 6256 70.6 95.2 27.5 99.2
a

true positive: positive in microbiological standard-of-care testing and positive in the multiplexed assay.

b

false negative: positive in microbiological standard-of-care testing and negative in the multiplexed assay.

c

false positive: negative in microbiological standard-of-care testing and positive in the multiplexed assay.

d

true negative: negative in microbiological standard-of-care testing and negative in the multiplexed assay.

e

positive predictive value.

f

negative predictive value [confirmed FN and FP in brackets].

* confirmation of Streptococcus: only as "Streptococcus spp."

Additional pathogens not covered by the multiplexed assay: 114 yeasts (including 85 Candida spp.), 7 other fungi, 9 Citrobacter spp., 49 coagulase neg. staphylococci, 34 enterococci, 35 streptococci (mostly viridans group), 8 other Gram-positive bacteria (Leuconostoc spp., Rothia spp., Corynebacterium spp.), 3 Pseudomonas spp., 10 Neisseria spp., 3 Haemophilus spp., 10 Citrobacter spp., 7 other Gram-negative bacteria (Ralstonia spp., Achromobacter spp., Burkholderia spp., Raoultella spp., Serratia spp.).