Table 3. Protein expression constructs.
RhoA | AKAP-Lbc DH–PH domain | AKAP-Lbc DH domain | |
---|---|---|---|
Residue range* | Met1–Gly184 | Lys1973–Asp2342 | Glu1976–Lys2211 |
Expression vector | pNIC28-Bsa4 | pNIC28-Bsa4 | pNIC28-Bsa4 |
Molecular mass (kDa) | 20.9 | 43.4 | 27.9 |
*Sequences of the protein constructs that were used can be found in the Supplementary Online Material.