Fig. 4.

Intervesicle transfer experiments using sterol-modified proteins 11 (black) and 12 (gray). Buffer solutions containing 5 μM POPC vesicles doped with 2 mol % Rho-DHPE were incubated with 50 nM protein–lipid chimera to generate lipoprotein-loaded POPC vesicles. After a stable baseline had been established a 12-fold excess of pure POPC vesicles was added (arrow) and the fluorescence change was monitored. All experiments were carried out at 20°C.