Fig. 5.

Titration of lipoproteins 11 and 12 with POPC/Rho-DHPE vesicles. Androstenol- and cholesterol-modified lipoproteins were diluted in buffer to a final concentration between 50 and 100 nM and the change in NBD emission at 535 nm was monitored after stepwise addition of POPC vesicles containing 4 mol % Rho-DHPE. (A) Raw data for titration of lipoprotein 11 (♦, left axis). Nonspecific increase in signal due to the addition of Rho-DHPE-containing vesicles was measured in a cuvette filled with buffer only (+, right axis). (B and C) Binding curves for lipoprotein 11 and 12, respectively.