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. 2016 Dec 24;28(12):5071–5082. doi: 10.1096/fj.14-255786

Figure 5.

Figure 5.

Subcellular localization of RIF NPs. MDMs were treated with 300 μM dye-labeled RIF NPs for 8 h. Cells were fixed with PFA and probed with antibodies to Rab 5 (A), Rab 7 (B), Rab 11 (C), or Rab 14 (D). Primary antibodies were detected with Alexa Fluor 488-labeled secondary antibody. Nanoparticles are shown in red, cell compartments in green, nuclei in blue, and overlay of the compartment and particle in yellow.