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. 2004 May 25;101(23):8551–8556. doi: 10.1073/pnas.0402889101

Fig. 2.

Fig. 2.

Myc-tagged SUMO and sumoylated proteins mainly localized in the nucleus. After Myc-SUMO stably expressed HEK 293 Tet-On cells were induced with 2 μg/ml Dox for 48 h, cells were fixed with 3.7% formaldehyde and stained with anti-Myc primary antibody and FITC-conjugated secondary antibody. DAPI was used to stain nuclei.