Table 1. Identified SUMO substrates.
| Protein name | GenBank accession number | Number of peptides identified* | Consensus sequence† | sumoplot score† |
|---|---|---|---|---|
| hnRNPs | ||||
| hnRNP A1 | X12671 | 2 | IKED | 0.9444 |
| hnRNP A3 | P51991 | 1 | IKED | 0.9444 |
| hnRNP F | NM_004966 | 1 | LKKD | 0.9056 |
| hnRNP H1 | NM_005520 | 7 | LKKD | 0.9056 |
| hnRNP K | NM_025279 | 2 | IKID | 0.9444 |
| hnRNP U | X65488 | 1 | LKEE | 0.9056 |
| Zinc finger proteins | ||||
| Zinc finger protein 226 | AF228418 | 3 | IKNE | 0.9444 |
| Zinc finger protein 6 | X56465 | 2 | IKTE | 0.9444 |
| Zinc finger protein 221 | AF187987 | 2 | FKCE | 0.8500 |
| Zinc finger protein 409 | AB028979 | 1 | IKEE | 0.9444 |
| Zinc finger protein 291 | AF242528 | 1 | IKKE | 0.9056 |
| Nuclear pore complex proteins | ||||
| Ran GTPase-activating protein 1 (RanGAP1)‡ | X82260 | 7 | LKSE | 0.9056 |
| Ran-binding protein 2 (RanBP2)‡ | S66431 | 19 | IKSE | 0.9444 |
| Ran-binding protein 16 (exportin 7) | AF064729 | 1 | VKVE | 0.9278 |
| Nuclear pore complex protein | AF071076 | 2 | FKAE | 0.8500 |
| Nup98-Nup96 | ||||
| Others | ||||
| Human DNA topoisomerase II, β* | X68060 | 1 | VKVE | 0.9278 |
| Sterol regulatory element-binding transcription factor* | NM_004599 | 1 | IKTD | 0.9444 |
| MAPK/ERK kinase kinase 1 | Q13233 | 2 | IKDE | 0.9444 |
| FUSE binding protein (FBP) | A53184 | 14 | FKPD | 0.8500 |
| FUSE binding protein 2 (FBP2) | U69126 | 2 | FKQD | 0.8500 |
MAPK/ERK, mitogen-activated protein kinase/extracellular signal-related kinase; FUSE, far upstream element.
*
The number of amino acid residues in identified peptides ranges from 10 to 25.
†
Sumoylation consensus sequence and theoretical score were generated by sumoplot software.
‡
Previously known SUMO substrates.