Table 1. Sequences of primers used for genomic PCR.
Transgenic mice | Forward primer sequence (5′ to 3′) | Reverse primer sequence (5′ to 3′) | Amplicon size (bp) |
AromKO | GTGACAGAGACATAAAGATCGAGGAT | AAATTCATTGGGCTTAGGGAAGTACTCG | 218 for WT |
GTGACAGAGACATAAAGATCGAGGAT | TCAGAGCAGCCGATTGTCTGTTGTGCCCAGTCAT | 410 for AromKO | |
αERKO | CGGTCTACGGCCAGTCGGGCATC | CAGGCCTTACACAGCGCCACCC | 400 for WT |
CGGTCTACGGCCAGTCGGGCATC | GCTGACCGCTTCCTCGTGCTTTAC | 750 for αERKO | |
βERKO | TGGACTCACCACGTAGGCTC | CATCCTTCACAGGACCAGACAC | 450 for WT |
TGGACTCACCACGTAGGCTC | GCAGCCTCTGTTCCACATACAC | 500 for βERKO | |
ARKO | CATTTGGGCCAGCTAAACAT | ATTCTCCCACCGTCAGTACG | 308 for Cre |
AAGATAAGCTTACATAATCACATGGA | CCTATGAAATCCTTTGCTGCACATGT | 814 for AR | |
AAGATAAGCTTACATAATCACATGGA | CCTATGAAATCCTTTGCTGCACATGT | 629 for ZFY-1 | |
AAGTGAATGGTCTTGGC | TTACAGGTCTGGTGCAAGCC | 400 or 430 for AR or floxed AR |