Table 2. Sequences of primers used for real-time PCR.

Target gene	Forward primer sequence (5′ to 3′)	Reverse primer sequence (5′ to 3′)	Amplicon size (bp)	NCBI reference sequence
AR	AAGCTGAAGAAACTTGGAAATCTAAA	TCAATGGCTTCCAGGACG	155	NM_013476
Aromatase	GAGGATGACGTAATTGACGG	AAAGGCTGAAAGTACCTGTAG	155	NM_007810.3
ERα	GTTGAAGTTTAATTGCTTGTTTATTGGAC	CATGACACGGTAGTTTGAAACG	156	NM_007956.4
ERβ	CTGTGATGAACTACAGTGTTCCC	GGTTCTGCATAGAGAAGCGAT	151	NM_207707.1, NM_010157.3

Primers for ERβ were designed to amplify both ERβ1 and ERβ2 cDNAs.