Figure 4. Six other mesenchymal cell lines were unable to attach to rPP.

Three hDPC (human dental pulp cells) cells from different donors, Saos2 (human osteosarcoma cell line), parental MC3T3-E1 (heterogeneous cell population), and C2C12 (mouse myoblast cell line) were seeded onto rPP, rC-DMP-1, and vitronectin (100 nM) with MnCl₂ (1 mM). The number of attached cells was evaluated as described before. Each value represents the mean of triplicate determinations; bars mean ±SD. Statistical analysis was performed by a one-way ANOVA, followed by Dunnett's test. ***p<0.001 indicates significantly lower and ⁺⁺p<0.01 and ⁺⁺⁺p<0.001 indicate significantly higher than rC-DMP-1-coated wells.