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. 2014 Nov 14;9(11):e112490. doi: 10.1371/journal.pone.0112490

Figure 6. The inability of rPP, rPP-ΔSSD, and rPP-RGE to bind to integrin αvβ3 or αvβ5.

Figure 6

(A) MG63 cells were preincubated with the neutralizing antibodies against integrin αvβ3 (LM609), αvβ5 (P1F6), and β1 (4B7) and control IgG (10 µg/ml) for 15 min, and were then seeded onto rC-DMP-1 and vitronectin (100 nM) in serum-free medium. Statistical analysis was performed by a one-way ANOVA, followed by Dunnett's test. **p<0.01 and ***p<0.001 indicate significantly lower than the cells preincubated with control IgG. (B) The binding of integrin αvβ3 and αvβ5 to rPP, rPP-ΔSSD, rPP-RGE, rC-DMP-1, and vitronectin was analyzed using solid phase binding assays. Various amounts (0∼400 ng) of integrin αvβ3 and αvβ5 were added to 96-well plates precoated with either 100 nM of PP, PP-ΔSSD, PP-RGE, rC-DMP-1, or vitronectin. Bound integrin αvβ3 and αvβ5 were then detected with the anti-integrin αv antibody, an appropriate HRP-conjugated secondary antibody, and TMB. Each value represents the mean of triplicate determinations; bars mean ±SD.