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. 2014 Nov 14;9(11):e112312. doi: 10.1371/journal.pone.0112312

Table 1. Leptospira strains used in the present study and results of the real time PCR assays using the species-specific probes and flanking primers.

Species Serogroup Serovar Strain Source1 Set 12 Set 23 Set 34 Set 45 Set 56
L. interrogans Australis Muenchen München C 90 KIT + + - - -
Australis Australis Ballico KIT + + - - -
Australis Bratislava Jez Bratislava INIAV + + - - -
Autumnalis Autumnalis Akiyami A INIAV + + - - -
Bataviae Bataviae Van Tienem INIAV + + - - -
Canicola Canicola Hond Utrecht IV INIAV + + - - -
Djasiman Djasiman Djasiman KIT + + - - -
Hebdomadis Hebdomadis Hebdomadis KIT + + - - -
Hebdomadis Kremastos Kremastos KIT + + - - -
Icterohaemorrhagiae Birkini Birkin KIT + + - - -
Icterohaemorrhagiae Copenhageni M20 INIAV + + - - -
Icterohaemorrhagiae Icterohaemorrhagiae RGA INIAV + + - - -
Icterohaemorrhagiae Lai Lai KIT + + - - -
Pomona Pomona Pomona INIAV + + - - -
Pyrogenes Pyrogenes Salinem INIAV + + - - -
Sejroe Hardjo type Prajitno Hardjoprajitno IHMT + + - - -
L. borgpetersenii Ballum Ballum Mus 127 INIAV + - + - -
Ballum Castellonis Castellon 3 KIT + - + - -
Hebdomadis Jules Jules KIT + - + - -
Hebdomadis Worsfoldi Worsfold KIT + - + - -
Javanica Ceylonica Piyasena KIT + - + - -
Javanica Poi Poi INIAV + - + - -
Javanica Zhenkang L 82 KIT + - + - -
Mini Mini Sari IHMT + - + - -
Pyrogenes Kwale Julu KIT + - + - -
Sejroe Hardjo type bovis Sponselee KIT + - + - -
Sejroe Hardjo type bovis L550 KIT + - + - -
Sejroe Hardjo type bovis JB197 KIT + - + - -
Sejroe Nyanza Kibos KIT + - + - -
Sejroe Sejroe M84 KIT + - + - -
Tarassovi Kisuba Kisuba KIT + - + - -
Tarassovi Tarassovi Mitis Johnson INIAV + - + - -
L. kirschneri Australis Ramisi Musa KIT + - - + -
Autumnalis Bulgarica Nicolaevo KIT + - - + -
Autumnalis Butembo Butembo KIT + - - + -
Cynopteri Cynopteri 3522C IHMT + - - + -
Grippotyphosa Grippotyphosa type Moskva Moskva V IHMT + - - + -
Grippotyphosa Ratnapura Wumalasena KIT + - - + -
Grippotyphosa Vanderhoedeni Kipod 179 KIT + - - + -
Icterohaemorrhagiae Bogvere LT 60-69 KIT + - - + -
Pomona Mozdok 5621 KIT + - - + -
Pomona Mozdok Portugal 1990 INIAV + - - + -
Pomona Tsaratsovo B 81/7 KIT + - - + -
L. noguchii Australis Nicaragua 1011 KIT + - - - +
Autumnalis Fortbragg Fort Bragg KIT + - - - +
Bataviae Argentiniensis Peludo KIT + - - - +
Djasiman Huallaga M 7 KIT + - - - +
Louisiana Louisiana LSU 1945 KIT + - - - +
Panama Panama CZ 214 INIAV + - - - +
Pomona Proechimys 1161 U KIT + - - - +
Pyrogenes Myocastoris LSU 1551 KIT + - - - +
Shermani Carimagua 9160 KIT + - - - +
L. santarosai Ballum Peru MW 10 KIT + - - - -
Bataviae Balboa 735 U KIT + - - - -
Bataviae Kobbe CZ 320 KIT + - - - -
Grippotyphosa Canalzonae CZ 188 KIT + - - - -
Hebdomadis Borincana HS 622 KIT + - - - -
Hebdomadis Maru CZ 285 KIT + - - - -
Javanica Fluminense Aa 3 KIT + - - - -
Mini Beye 1537 U KIT + - - - -
Sarmin Rio Rr 5 KIT + - - - -
Sejroe Guaricura Bov.G. KIT + - - - -
Shermani Babudieri CI 40 KIT + - - - -
Shermani Shermani 1342 K KIT + - - - -
Tarassovi Atchafalaya LSU 1013 KIT + - - - -
L. weilii Celledoni Celledoni Celledoni INIAV + - - - -
Celledoni Mengding M 6906 KIT + - - - -
Javanica Coxi Cox KIT + - - - -
Javanica Mengma S 590 KIT + - - - -
Javanica Mengrun A 102 KIT + - - - -
Mini Hekou H 27 KIT + - - - -
Pyrogenes Menglian S 621 KIT + - - - -
Sarmin Sarmin Sarmin KIT + - - - -
Tarassovi Topaz 94-79970/3 KIT + - - - -
Tarassovi Vughia LT 89-68 KIT + - - - -
L. alexanderi Hebdomadis Manzhuang A 23 KIT nd - - - -
Javanica Mengla A 85 KIT nd - - - -
Manhao Manhao 3 L 60 KIT nd - - - -
Mini Yunnan A 10 KIT nd - - - -
L. meyeri Ranarum Ranarum ICF KIT nd - - - -
Semaranga Semaranga Veldrat Semaranga 173 KIT nd - - - -
L. inadai Manhao Lincang L 14 KIT nd - - - -
L.fainei Hurstbridge Hurstbridge BUT 6T KIT nd - - - -
L.biflexa Andaman Andamana CH 11 KIT - - - - -
Semaranga Patoc Patoc I KIT - - - - -
1

INIAV - Instituto Nacional de Investigação Agrária e Veterinária, Lisbon, Portugal. IHMT - Instituto de Higiene e Medicina Tropical, Lisbon, Portugal. KIT - Royal Tropical Institute, Amsterdam, The Netherlands;

2

Set 1 targets the lipL32 gene of pathogenic Leptospira spp.;

3

Set 2 targets the secY gene of L. interrogans;

4

Set 3 targets the ompL1 gene of L. borgpetersenii;

5

Set 4 targets the secY gene of L. kirschneri;

6

Set 5 targets the secY gene of L. noguchii; nd - not done; Amplification (+) or no amplification (−).