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. 2014 Nov 14;9(11):e112312. doi: 10.1371/journal.pone.0112312

Table 2. Results of the bacteriological culture and of the real time amplification assays for the tissue samples analyzed in the present study.

Sample Origin Set Actin1 Set 12 Set 23 Set 34 Set 45 Set 56 Bacteriological analysis7
12-17433-Z1 Mus sp. + + - + - - L. borgpetersenii
12-18078-Z6 Mus sp. + + - + - - L. borgpetersenii
12-18458-Z13 Mus sp. + - - - - - Negative
12-18458-Z14 Mus sp. + - - - - - Negative
12-19472-Z15 Mus sp. + - - - - - Negative
12-20553-Z16 Mus sp. + - - - - - Negative
12-22955-Z17 Mus sp. + - - - - - Negative
12-22955-Z18 Mus sp. + - - - - - Negative
12-22955-Z19 Mus sp. + - - - - - Negative
12-22955-Z20 Mus sp. + - - - - - Negative
12-22955-Z22 Mus sp. + - - - - - Negative
12-22955-Z23 Mus sp. + - - - - - Negative
12-22955-Z24 Mus sp. + - - - - - Negative
12-22955-Z25 Mus sp. + - - - - - Negative
12-22955-Z26 Mus sp. + - - - - - Negative
12-22955-Z27 Mus sp. + - - - - - Negative
12-22955-Z28 Mus sp. + - - - - - Negative
12-22955-Z29 Mus sp. + - - - - - Negative
12-22955-Z30 Mus sp. + - - - - - Negative
12-22955-Z31 Mus sp. + - - - - - Negative
12-22955-Z32 Mus sp. + - - - - - Negative
12-22955-Z33 Mus sp. + - - - - - Negative
12-22955-Z34 Mus sp. + - - - - - Negative
12-22955-Z36 Mus sp. + - - - - - Negative
12-22955-Z37 Rattus sp. + + + - - - L. interrogans
12-22955-Z38 Mus sp. + - - - - - Negative
12-22955-Z39 Rattus sp. + - - - - - Negative
11-36840 Dolichotis patagonum + + + - - - L. interrogans
12-494 Sus domesticus (fetus) + + - - + - L. kirschneri
1

Set Actin targets the β-actin gene of mammals,

2

Set 1 targets the lipL32 gene of pathogenic Leptospira;

3

Set 2 targets the secY gene of L. interrogans;

4

Set 3 targets the ompL1 gene of L. borgpetersenii;

5

Set 4 targets the secY gene of L. kirschneri;

6

Set 5 targets the secY gene of L. noguchii;

7

The analysis of the partial sequences of the secY gene of each isolate allowed to identify the Leptospira species; Amplification (+) or no amplification (−).